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recombinant mouse gas6 protein  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse gas6 protein
    Recombinant Mouse Gas6 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+gas6/Recombinant+Mouse+Gas6+Protein/pm41885342-31-18-22
    Average 95 stars, based on 56 article reviews
    recombinant mouse gas6 protein - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Anti-Axl antagonistic antibodies
    Article Snippet: The cell lysates were prepared, as described in EXAMPLE 9, the Western blot analysis was performed using anti-phospho-Akt (Ser473) antibody (Cell Signaling) followed by goat anti-rabbit horseradish peroxidase (Jackson ImmunoResearch); the anti-phospho-Akt does not distinguish between AKT1, AKT2, and AKT3, hence the total level of ‘phospho-Akt’ is shown in the blot.

    Functional Assay:

    Article Title: GLP-1R activation restores Gas6-driven efferocytosis in senescent foamy macrophages to promote neural repair
    Article Snippet: .. For functional assays, BMDMs were pretreated with either the p53-specific inhibitor Pifithrin-β (PFT-β, 10 μM for 24 h; HY-16702A, MedChemExpress), Exendin-4 (Ex-4, 100, 200, or 400 nM for 24 h; HY-13443, MedChemExpress), compound 5d (1 μM for 24 h, HY-101116, MedChemExpress), recombinant Mouse Gas6 (rGas6, 500 ng/mL for 2h; 986-GS-025/CF, R&D Systems, Minneapolis, MN, USA) or BML-275 (1 μM for 24 h; HY-13418A, MedChemExpress), followed by stimulation with MD (1 mg/mL) for 12 h. .. SA-β-gal staining was performed using a β-Galactosidase Staining Kit (C0602, Beyotime, China) following the manufacturer's instructions.

    Article Title: GLP-1R activation restores Gas6-driven efferocytosis in senescent foamy macrophages to promote neural repair.
    Article Snippet: .. For functional assays, BMDMs were pretreated with either the p53-specific inhibitor Pifithrin-β (PFT-β, 10 μM for 24 h; HY-16702A, MedChemExpress), Exendin-4 (Ex4, 100, 200, or 400 nM for 24 h; HY-13443, MedChemExpress), compound 5d (1 μM for 24 h, HY-101116, MedChemExpress), recombinant Mouse Gas6 (rGas6, 500 ng/mL for 2h; 986-GS-025/CF, R&D Systems, Minneapolis, MN, USA) or BML-275 (1 μM for 24 h; HY-13418A, MedChemExpress), followed by stimulation with MD (1 mg/mL) for 12 h. .. SA-β-gal staining was performed using a β-Galactosidase Staining Kit (C0602, Beyotime, China) following the manufacturer’s instructions.

    Recombinant:

    Article Title: GLP-1R activation restores Gas6-driven efferocytosis in senescent foamy macrophages to promote neural repair
    Article Snippet: .. For functional assays, BMDMs were pretreated with either the p53-specific inhibitor Pifithrin-β (PFT-β, 10 μM for 24 h; HY-16702A, MedChemExpress), Exendin-4 (Ex-4, 100, 200, or 400 nM for 24 h; HY-13443, MedChemExpress), compound 5d (1 μM for 24 h, HY-101116, MedChemExpress), recombinant Mouse Gas6 (rGas6, 500 ng/mL for 2h; 986-GS-025/CF, R&D Systems, Minneapolis, MN, USA) or BML-275 (1 μM for 24 h; HY-13418A, MedChemExpress), followed by stimulation with MD (1 mg/mL) for 12 h. .. SA-β-gal staining was performed using a β-Galactosidase Staining Kit (C0602, Beyotime, China) following the manufacturer's instructions.

    Article Title: Gas6/Axl signaling promotes hematoma resolution and motivates protective microglial responses after intracerebral hemorrhage in mice.
    Article Snippet: Background: Intracerebral hemorrhage (ICH) stands out as the most fatal subtype of stroke, currently devoid of effective therapy.. Recent research underscores the significance of Axl and its ligand growth arrest-specific 6 (Gas6) in normal brain function and a spectrum of neurological disorders, including ICH.. This study is designed to delve into the role of Gas6/Axl signaling in facilitating hematoma clearance and neuroinflammation resolution

    Article Title: Anti-Axl antagonistic antibodies
    Article Snippet: .. The cells were pre-incubated with anti-Axl antibody 10G5 at concentration 1 μg/mL for 1 hr followed by stimulation with Axl ligand, recombinant mouse Gas6 (R&D Systems), at concentration 10 μg/mL for 20 min. .. The cell lysates were prepared, as described in EXAMPLE 9, the Western blot analysis was performed using anti-phospho-Akt (Ser473) antibody (Cell Signaling) followed by goat anti-rabbit horseradish peroxidase (Jackson ImmunoResearch); the anti-phospho-Akt does not distinguish between AKT1, AKT2, and AKT3, hence the total level of ‘phospho-Akt’ is shown in the blot.

    Article Title: Modulation of microglial phagocytosis via the GAS6-MERTK pathway regulates pathological angiogenesis in the mouse oxygen-induced retinopathy model
    Article Snippet: Intravitreal injections were performed with a 33-gauge needle (Hamilton, Bonaduz, Switzerland) under a surgical microscope in anesthetized mice on P14 of the OIR model [ ]. .. Mice received 100 ng of either recombinant mouse MerTK-FC (R&D systems, Minneapolis, Minnesota, USA), recombinant mouse GAS6 (R&D systems), or recombinant mouse Milk fat globule-EGF factor 8 protein (MFGE8; R&D systems) into the right eye. ..

    Article Title: GLP-1R activation restores Gas6-driven efferocytosis in senescent foamy macrophages to promote neural repair.
    Article Snippet: .. For functional assays, BMDMs were pretreated with either the p53-specific inhibitor Pifithrin-β (PFT-β, 10 μM for 24 h; HY-16702A, MedChemExpress), Exendin-4 (Ex4, 100, 200, or 400 nM for 24 h; HY-13443, MedChemExpress), compound 5d (1 μM for 24 h, HY-101116, MedChemExpress), recombinant Mouse Gas6 (rGas6, 500 ng/mL for 2h; 986-GS-025/CF, R&D Systems, Minneapolis, MN, USA) or BML-275 (1 μM for 24 h; HY-13418A, MedChemExpress), followed by stimulation with MD (1 mg/mL) for 12 h. .. SA-β-gal staining was performed using a β-Galactosidase Staining Kit (C0602, Beyotime, China) following the manufacturer’s instructions.

    Article Title: Anti-axl antagonistic antibodies
    Article Snippet: .. The cells were pre-incubated with anti-Axl antibody 10G5 at concentration 1 μg/mL for 1 hr followed by stimulation with Axl ligand, recombinant mouse Gas6 (R&D Systems), at concentration 10 μg/mL for 20 min. .. The cell lysates were prepared, as described in EXAMPLE 9, the Western blot analysis was performed using anti-phospho-Akt (Ser473) antibody (Cell Signaling) followed by goat anti-rabbit horseradish peroxidase (Jackson ImmunoResearch); the anti-phospho-Akt does not distinguish between AKT1, AKT2, and AKT3, hence the total level of ‘phospho-Akt’ is shown in the blot.

    Article Title: Modulation of microglial phagocytosis via the GAS6-MERTK pathway regulates pathological angiogenesis in the mouse oxygen-induced retinopathy model.
    Article Snippet: Intravitreal injections were performed with a 33- gauge needle (Hamilton, Bonaduz, Switzerland) under a surgical microscope in anesthetized mice on P14 of the OIR model [14]. .. Mice received 100 ng of either recombinant mouse MerTK-FC (R&D systems, Minneapolis, Minnesota, USA), recombinant mouse GAS6 (R&D systems), or recombinant mouse Milk fat globule-EGF factor 8 protein (MFGE8; R&D systems) into the right eye. ..

    Concentration Assay:

    Article Title: Anti-Axl antagonistic antibodies
    Article Snippet: .. The cells were pre-incubated with anti-Axl antibody 10G5 at concentration 1 μg/mL for 1 hr followed by stimulation with Axl ligand, recombinant mouse Gas6 (R&D Systems), at concentration 10 μg/mL for 20 min. .. The cell lysates were prepared, as described in EXAMPLE 9, the Western blot analysis was performed using anti-phospho-Akt (Ser473) antibody (Cell Signaling) followed by goat anti-rabbit horseradish peroxidase (Jackson ImmunoResearch); the anti-phospho-Akt does not distinguish between AKT1, AKT2, and AKT3, hence the total level of ‘phospho-Akt’ is shown in the blot.

    Article Title: Anti-axl antagonistic antibodies
    Article Snippet: .. The cells were pre-incubated with anti-Axl antibody 10G5 at concentration 1 μg/mL for 1 hr followed by stimulation with Axl ligand, recombinant mouse Gas6 (R&D Systems), at concentration 10 μg/mL for 20 min. .. The cell lysates were prepared, as described in EXAMPLE 9, the Western blot analysis was performed using anti-phospho-Akt (Ser473) antibody (Cell Signaling) followed by goat anti-rabbit horseradish peroxidase (Jackson ImmunoResearch); the anti-phospho-Akt does not distinguish between AKT1, AKT2, and AKT3, hence the total level of ‘phospho-Akt’ is shown in the blot.

    Mouse Assay:

    Article Title: Modulation of microglial phagocytosis via the GAS6-MERTK pathway regulates pathological angiogenesis in the mouse oxygen-induced retinopathy model
    Article Snippet: Intravitreal injections were performed with a 33-gauge needle (Hamilton, Bonaduz, Switzerland) under a surgical microscope in anesthetized mice on P14 of the OIR model [ ]. .. Mice received 100 ng of either recombinant mouse MerTK-FC (R&D systems, Minneapolis, Minnesota, USA), recombinant mouse GAS6 (R&D systems), or recombinant mouse Milk fat globule-EGF factor 8 protein (MFGE8; R&D systems) into the right eye. ..

    Article Title: Modulation of microglial phagocytosis via the GAS6-MERTK pathway regulates pathological angiogenesis in the mouse oxygen-induced retinopathy model.
    Article Snippet: Intravitreal injections were performed with a 33- gauge needle (Hamilton, Bonaduz, Switzerland) under a surgical microscope in anesthetized mice on P14 of the OIR model [14]. .. Mice received 100 ng of either recombinant mouse MerTK-FC (R&D systems, Minneapolis, Minnesota, USA), recombinant mouse GAS6 (R&D systems), or recombinant mouse Milk fat globule-EGF factor 8 protein (MFGE8; R&D systems) into the right eye. ..

    Milk:

    Article Title: Modulation of microglial phagocytosis via the GAS6-MERTK pathway regulates pathological angiogenesis in the mouse oxygen-induced retinopathy model
    Article Snippet: Intravitreal injections were performed with a 33-gauge needle (Hamilton, Bonaduz, Switzerland) under a surgical microscope in anesthetized mice on P14 of the OIR model [ ]. .. Mice received 100 ng of either recombinant mouse MerTK-FC (R&D systems, Minneapolis, Minnesota, USA), recombinant mouse GAS6 (R&D systems), or recombinant mouse Milk fat globule-EGF factor 8 protein (MFGE8; R&D systems) into the right eye. ..

    Article Title: Modulation of microglial phagocytosis via the GAS6-MERTK pathway regulates pathological angiogenesis in the mouse oxygen-induced retinopathy model.
    Article Snippet: Intravitreal injections were performed with a 33- gauge needle (Hamilton, Bonaduz, Switzerland) under a surgical microscope in anesthetized mice on P14 of the OIR model [14]. .. Mice received 100 ng of either recombinant mouse MerTK-FC (R&D systems, Minneapolis, Minnesota, USA), recombinant mouse GAS6 (R&D systems), or recombinant mouse Milk fat globule-EGF factor 8 protein (MFGE8; R&D systems) into the right eye. ..



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